Reference SummaryPond AC, Cancer Res 2010 Jun 15;70(12):4868-79

Title

Fibroblast growth factor receptor signaling dramatically accelerates tumorigenesis and enhances oncoprotein translation in the mouse mammary tumor virus-Wnt-1 mouse model of breast cancer.

Authors

Pond AC; Herschkowitz JI; Schwertfeger KL; Welm B; Zhang Y; York B; Cardiff RD; Hilsenbeck S; Perou CM; Creighton CJ; Lloyd RE; Rosen JM

Journal

Cancer Res

Volume

70

Issue

12

Year

2010

Pages

4868-79

Abstract

Fibroblast growth factor (FGF) cooperates with the Wnt/beta-catenin pathway to promote mammary tumorigenesis. To investigate the mechanisms involved in FGF/Wnt cooperation, we genetically engineered a model of inducible FGF receptor (iFGFR) signaling in the context of the well-established mouse mammary tumor virus-Wnt-1 transgenic mouse. In the bigenic mice, iFGFR1 activation dramatically enhanced mammary tumorigenesis. Expression microarray analysis did not show transcriptional enhancement of Wnt/beta-catenin target genes but instead showed a translational gene signature that also correlated with elevated FGFR1 and FGFR2 in human breast cancer data sets. Additionally, iFGFR1 activation enhanced recruitment of RNA to polysomes, resulting in a marked increase in protein expression of several different Wnt/beta-catenin target genes. FGF pathway activation stimulated extracellular signal-regulated kinase and the phosphorylation of key translation regulators both in vivo in the mouse model and in vitro in a human breast cancer cell line. Our results suggest that cooperation of the FGF and Wnt pathways in mammary tumorigenesis is based on the activation of protein translational pathways that result in, but are not limited to, increased expression of Wnt/beta-catenin target genes (at the level of protein translation). Further, they reveal protein translation initiation factors as potential therapeutic targets for human breast cancers with alterations in FGF signaling.

Links

J:160915 – MGI References
20501844 – National Library of Medicine/PubMed

Strain Notes

Strain Note
FVB-Tg(MMTV-Fgfr1)4775Jmr Tg(Wnt1)1Hev "All mice were FVB background littermates."
FVB-Tg(MMTV-Fgfr2)#Jmr Tg(Wnt1)1Hev "All mice were FVB background littermates."
FVB-Tg(Wnt1)1Hev "All mice were FVB background littermates."

Models

Strain Model Name Treatment Agent(s) Organ Affected Frequency Model Details
FVB-Tg(Wnt1)1Hev Mammary gland hyperplasia Mammary gland

observed

FVB-Tg(MMTV-Fgfr1)4775Jmr Tg(Wnt1)1Hev Mammary gland hyperplasia Mammary gland

observed

FVB-Tg(MMTV-Fgfr1)4775Jmr Tg(Wnt1)1Hev Mammary gland lesion - nodular
  • AP20187
Mammary gland

observed

FVB-Tg(MMTV-Fgfr2)#Jmr Tg(Wnt1)1Hev Mammary gland tumor Mammary gland

observed

FVB-Tg(MMTV-Fgfr1)4775Jmr Tg(Wnt1)1Hev Mammary gland tumor - solid
  • AP20187
Mammary gland

100

FVB-Tg(Wnt1)1Hev Mammary gland tumor - type P
  • AP20187
Mammary gland

52