Reference SummaryRyan J, J Natl Cancer Inst 1987 Dec;79(6):1351-7

Title

 KRAS2 as a genetic marker for lung tumor susceptibility in inbred mice.

Authors

 Ryan J; Barker PE; Nesbitt MN; Ruddle FH

Journal

 J Natl Cancer Inst

Volume

 79

Issue

 6

Year

 1987

Pages

 1351-7

Abstract

 An Eco-RI restriction fragment length polymorphism occurring in a DNA fragment containing the first exon of the murine KRAS2 gene was shown to correlate with the inherited susceptibility of inbred strains of mice to urethan (CAS: 51-79-6)-induced pulmonary adenomas. Eco-RI digestion of murine DNA yielded four KRAS2-specific fragments. Polymorphic variation occurred in the smallest molecular-weight fragment with alleles of either 0.70 or 0.55 kb in size. Genotyping of 14 inbred strains of mice revealed a correlation between KRAS2 Eco-RI polymorphic variation and the differential susceptibility among inbred strains to development of pulmonary adenomas. Strains with a high incidence of pulmonary adenomas, either spontaneously occurring or in response to carcinogen induction, had the 0.55-kb KRAS2 allele whereas adenoma-resistant strains had the 0.70-kb allele. Analysis of a series of recombinant inbred strains (AXB, BXA) that developed from reciprocal crosses between a highly susceptible strain (A/J) and a highly resistant strain (C57BL/6J) revealed a statistically significant threefold difference in lung tumor susceptibility on the basis of KRAS2 genotype. Further analysis of individual F2 mice of a C57BL/6 female X A/J male cross also demonstrated a threefold difference in tumor susceptibility on the basis of KRAS2 allelic variation.

Links

 J:17778 – MGI References
2891865 – National Library of Medicine/PubMed

Strain Notes

Strain Note
(C57BL/6J x A/J)F2 Because these mice are F2 hybrids individual mice may show an individual mixture of alleles of genes from the parental strains.
C57BL/6J x B6AF1 Because these mice are a backcross in progress individual mice may show an individual mixture of alleles of genes from the parental strains.

Models
Strain Model Name Treatment Agent(s) Organ Affected Frequency Model Details
AXB1 Lung adenoma
  • urethane (ethyl carbamate)
 Lung

observed
AXB2 Lung adenoma
  • urethane (ethyl carbamate)
 Lung

observed
AXB3 Lung adenoma
  • urethane (ethyl carbamate)
 Lung

observed
AXB4 Lung adenoma
  • urethane (ethyl carbamate)
 Lung

observed
AXB5 Lung adenoma
  • urethane (ethyl carbamate)
 Lung

observed
AXB6 Lung adenoma
  • urethane (ethyl carbamate)
 Lung

observed
AXB7 Lung adenoma
  • urethane (ethyl carbamate)
 Lung

observed
AXB8 Lung adenoma
  • urethane (ethyl carbamate)
 Lung

observed
AXB9 Lung adenoma
  • urethane (ethyl carbamate)
 Lung

observed
AXB10 Lung adenoma
  • urethane (ethyl carbamate)
 Lung

observed
AXB11 Lung adenoma
  • urethane (ethyl carbamate)
 Lung

observed
AXB12 Lung adenoma
  • urethane (ethyl carbamate)
 Lung

observed
AXB13 Lung adenoma
  • urethane (ethyl carbamate)
 Lung

observed
AXB14 Lung adenoma
  • urethane (ethyl carbamate)
 Lung

observed
AXB15 Lung adenoma
  • urethane (ethyl carbamate)
 Lung

observed
AXB17 Lung adenoma
  • urethane (ethyl carbamate)
 Lung

observed
AXB19 Lung adenoma
  • urethane (ethyl carbamate)
 Lung

observed
AXB20 Lung adenoma
  • urethane (ethyl carbamate)
 Lung

observed
AXB21 Lung adenoma
  • urethane (ethyl carbamate)
 Lung

observed
AXB22 Lung adenoma
  • urethane (ethyl carbamate)
 Lung

observed
AXB23 Lung adenoma
  • urethane (ethyl carbamate)
 Lung

observed
AXB24 Lung adenoma
  • urethane (ethyl carbamate)
 Lung

observed
BXA1 Lung adenoma
  • urethane (ethyl carbamate)
 Lung

observed
BXA2 Lung adenoma
  • urethane (ethyl carbamate)
 Lung

observed
BXA4 Lung adenoma
  • urethane (ethyl carbamate)
 Lung

observed
BXA8 Lung adenoma
  • urethane (ethyl carbamate)
 Lung

observed
BXA9 Lung adenoma
  • urethane (ethyl carbamate)
 Lung

observed
BXA10 Lung adenoma
  • urethane (ethyl carbamate)
 Lung

observed
BXA12 Lung adenoma
  • urethane (ethyl carbamate)
 Lung

observed
BXA13 Lung adenoma
  • urethane (ethyl carbamate)
 Lung

observed
BXA14 Lung adenoma
  • urethane (ethyl carbamate)
 Lung

observed
BXA16 Lung adenoma
  • urethane (ethyl carbamate)
 Lung

observed
BXA17 Lung adenoma
  • urethane (ethyl carbamate)
 Lung

observed
BXA18 Lung adenoma
  • urethane (ethyl carbamate)
 Lung

observed
BXA19 Lung adenoma
  • urethane (ethyl carbamate)
 Lung

observed
BXA20 Lung adenoma
  • urethane (ethyl carbamate)
 Lung

observed
BXA22 Lung adenoma
  • urethane (ethyl carbamate)
 Lung

observed
BXA23 Lung adenoma
  • urethane (ethyl carbamate)
 Lung

observed
BXA24 Lung adenoma
  • urethane (ethyl carbamate)
 Lung

observed
BXA25 Lung adenoma
  • urethane (ethyl carbamate)
 Lung

observed
(C57BL/6J x A/J)F2 Lung adenoma
  • urethane (ethyl carbamate)
 Lung

observed
C57BL/6J x B6AF1 Lung adenoma
  • urethane (ethyl carbamate)
 Lung

observed
A/J Lung tumor Lung

90
A/HeJ Lung tumor Lung

90
SWR/J Lung tumor Lung

90
BALB/cJ Lung tumor Lung

40.0 - 50.0
CBA/J Lung tumor Lung

20
RF/J Lung tumor Lung

40
C3H/HeJ Lung tumor Lung

5.0 - 10.0
C57L/J Lung tumor Lung

1
C57BL/6J Lung tumor Lung

1
DBA/2J Lung tumor Lung

5.0 - 10.0
SJL/J Lung tumor Lung

5
AKR/J Lung tumor Lung

5