Reference SummaryThompson JA, Int J Cancer 1997 Jul 3;72(1):197-202

Title

Expression of transgenic carcinoembryonic antigen (CEA) in tumor-prone mice: an animal model for CEA-directed tumor immunotherapy.

Authors

Thompson JA; Eades-Perner AM; Ditter M; Muller WJ; Zimmermann W

Journal

Int J Cancer

Volume

Issue

Year

1997

Pages

197-202

Abstract

Carcinoembryonic antigen (CEA) is a tumor marker for the most common forms of adenocarcinomas, We have previously described C57BL/6 mice transgenic for the complete human CEA gene, Compared with humans, these mice reveal a conserved spatiotemporal CEA expression pattern, To establish animal models for CEA-targeted tumor immunotherapy, we have crossed CEA transgenic mice with mice that are genetically predisposed to tumor development, These immunocompetent animals should allow optimization of immunotherapy strategies for maximal destruction of tumor tissues with minimal damage to CEA- expressing normal tissues, To develop a breast tumor model, CEA transgenic mice were cross-bred with mice transgenic for the rat neo protooncogene controlled by the mouse mammary tumor virus long terminal repeat, Female offspring developed poorly differentiated breast tumors, none of which, however, expressed CEA, As a model for colorectal tumors, mice bearing a mutation in the Ape gene (Min mice) and the CEA transgene developed multiple intestinal adenomas with strong CEA expression in all tumor cells, CEA expression had no significant effect on tumor growth, Occasional, well-differentiated breast adenocarcinomas in female offspring expressed CEA focally in tumor cells lining pseudolumina, Cross-breeding Apc(Min/+) mice with neu transgenic mice did not reveal a synergistic effect on the kinetics of breast tumor formation, Finally, CEA transgenic mice crossbred with mice transgenic for the SV40 large T antigen regulated by the surfactant protein-C promoter, developed multiple lung adenocavcinomas that revealed a mosaic CEA expression pattern. (C) 1997 Wiley-Liss, Inc.

Links

J:41380 – MGI References
9212243 – National Library of Medicine/PubMed

Strain Notes

Strain	Note
STOCK	Mice were generated by mating Tg(CEACAM5)#Wzm mice from 1st or 2nd filial backcross onto C57BL/6 with C57BL/6J-Apc^Min/J mice purchased from The Jackson Laboratory.
STOCK Apc^Min/+	Mice were generated by mating Tg(CEACAM5)#Wzm mice from 1st or 2nd filial backcross onto C57BL/6 with C57BL/6J-Apc^Min/J mice purchased from The Jackson Laboratory.
STOCK Apc^Min/+ Tg(CEACAM5)#Wzm	Mice were generated by mating Tg(CEACAM5)#Wzm mice from 1st or 2nd filial backcross onto C57BL/6 with C57BL/6J-Apc^Min/J mice purchased from The Jackson Laboratory.
STOCK Tg(CEACAM5)#Wzm	Mice were generated by mating Tg(CEACAM5)#Wzm mice from 1st or 2nd filial backcross onto C57BL/6 with C57BL/6J-Apc^Min/J mice purchased from The Jackson Laboratory.
STOCK Tg(CEACAM5)#Wzm Tg(MMTVneu)202Mul	Mice were generated by mating Tg(CEACAM5)#Wzm mice from 1st or 2nd filial backcross onto C57BL/6 with FVB/N-Tg(MMTVneu)202Mul mice.
STOCK Tg(CEACAM5)#Wzm Tg(SFTPC-TAg)5.1Jaw	Mice were generated by mating Tg(CEACAM5)#Wzm mice from 1st or 2nd filial backcross onto C57BL/6 with FVB/N-Tg(SFTPC-TAg)5Jaw mice provided by Dr. J. Whitsett at the Children's Hospital Medical Center, Cincinatti, OH.
STOCK Tg(SFTPC-TAg)5.1Jaw	Mice were generated by mating Tg(CEACAM5)#Wzm mice from 1st or 2nd filial backcross onto C57BL/6 with FVB/N-Tg(SFTPC-TAg)5Jaw mice provided by Dr. J. Whitsett at the Children's Hospital Medical Center, Cincinatti, OH.

Models
Strain	Model Name	Organ Affected	Frequency
STOCK Apc^Min/+ Tg(CEACAM5)#Wzm	Intestine - Large Intestine - Colon adenoma	Intestine - Large Intestine - Colon	observed
STOCK Apc^Min/+	Intestine - Large Intestine - Colon adenoma	Intestine - Large Intestine - Colon	observed
STOCK Tg(CEACAM5)#Wzm	Intestine - Large Intestine - Colon adenoma	Intestine - Large Intestine - Colon	0
STOCK	Intestine - Large Intestine - Colon adenoma	Intestine - Large Intestine - Colon	0
STOCK Apc^Min/+ Tg(CEACAM5)#Wzm	Intestine - Small Intestine adenoma	Intestine - Small Intestine	observed
STOCK Apc^Min/+	Intestine - Small Intestine adenoma	Intestine - Small Intestine	observed
STOCK Tg(CEACAM5)#Wzm	Intestine - Small Intestine adenoma	Intestine - Small Intestine	0
STOCK	Intestine - Small Intestine adenoma	Intestine - Small Intestine	0
STOCK Apc^Min/+ Tg(CEACAM5)#Wzm Tg(MMTVneu)202Mul	Intestine adenoma	Intestine	observed
C57BL/6J-Apc^Min/+	Intestine adenoma	Intestine	observed
STOCK Tg(CEACAM5)#Wzm Tg(SFTPC-TAg)5.1Jaw	Lung adenocarcinoma	Lung	observed
STOCK Tg(CEACAM5)#Wzm Tg(SFTPC-TAg)5.1Jaw	Lung adenoma	Lung	observed
STOCK Tg(SFTPC-TAg)5.1Jaw	Lung adenoma	Lung	observed
STOCK Tg(CEACAM5)#Wzm Tg(SFTPC-TAg)5.1Jaw	Lung hyperplasia	Lung	observed
STOCK Apc^Min/+	Mammary gland adenoma	Mammary gland	observed
STOCK Apc^Min/+ Tg(CEACAM5)#Wzm	Mammary gland tumor	Mammary gland	18
STOCK Tg(CEACAM5)#Wzm Tg(MMTVneu)202Mul	Mammary gland tumor	Mammary gland	observed
STOCK Apc^Min/+ Tg(CEACAM5)#Wzm Tg(MMTVneu)202Mul	Mammary gland tumor	Mammary gland	observed