Reference SummaryGuy CT, Proc Natl Acad Sci U S A 1992 Nov 15;89(22):10578-82

Title

 Expression of the neu protooncogene in the mammary epithelium of transgenic mice induces metastatic disease.

Authors

 Guy CT; Webster MA; Schaller M; Parsons TJ; Cardiff RD; Muller WJ

Journal

 Proc Natl Acad Sci U S A

Volume

 89

Issue

 22

Year

 1992

Pages

 10578-82

Abstract

 Overexpression and amplification of the neu (c-erbB2, ERBB2) protooncogene have been implicated in the development of aggressive human breast cancer. To directly assess the effect of mammary gland- specific expression of the neu protooncogene, transgenic mice carrying unactivated neu under the transcriptional control of the mouse mammary tumor virus promoter/enhancer were established. By contrast to the rapid tumor progression observed in several transgenic strains carrying the activated neu transgene, expression of unactivated neu in the mammary epithelium resulted in the development of focal mammary tumors after long latency. The majority of the mammary tumors analyzed expressed elevated levels of neu-encoded mRNA and protein. Overexpression of neu in the mammary tumors was also associated with elevated neu intrinsic tyrosine kinase activity and the de novo tyrosine phosphorylation of several cellular proteins. Interestingly, many of the tumor-bearing transgenic mice developed secondary metastatic tumors in the lung. These observations suggest that overexpression of the unactivated neu protein can induce metastatic disease after long latency.

Links

 J:56332 – MGI References
1359541 – National Library of Medicine/PubMed

Strain Notes

Strain Note
FVB/N-Tg(MMTVneu)169Mul Expression of the "MMTVneu" transgene was reported to not be detected in the lungs of these mice.
The "MMTVneu" transgene was reported to be expressed in the mammary glands and salivary glands of these mice.
FVB/N-Tg(MMTVneu)202Mul The "MMTVneu" transgene was reported to be expressed in the mammary glands, salivary glands, and lungs of these mice.
The wild type allele of the Rpl32 gene was reported to be expressed in the mammary glands of these mice.
FVB/N-Tg(MMTVneu)510Mul Expression of the "MMTVneu" transgene was reported to not be detected in the lungs of these mice.
The "MMTVneu" transgene was reported to be expressed in the mammary glands and salivary glands of these mice.
FVB/N-Tg(MMTVneu)721Mul Expression of the "MMTVneu" transgene was reported to not be detected in the lungs of these mice.
The "MMTVneu" transgene was reported to be expressed in the mammary glands and salivary glands of these mice.
FVB/N-Tg(MMTVneu)732Mul Expression of the "MMTVneu" transgene was reported to not be detected in the lungs of these mice.
The "MMTVneu" transgene was reported to be expressed in the mammary glands and salivary glands of these mice.

Models
Strain Model Name Treatment Agent(s) Organ Affected Frequency Model Details
FVB/N-Tg(MMTVneu)202Mul Mammary gland adenocarcinoma Mammary gland

observed - 70
FVB/N-Tg(MMTVneu)169Mul Mammary gland adenocarcinoma Mammary gland

50
FVB/N-Tg(MMTVneu)510Mul Mammary gland adenocarcinoma Mammary gland

50
FVB/N-Tg(MMTVneu)721Mul Mammary gland adenocarcinoma Mammary gland

50
FVB/N-Tg(MMTVneu)732Mul Mammary gland adenocarcinoma Mammary gland

50