Reference SummaryCalzada-Wack J, Carcinogenesis 2002 May;23(5):727-33
Title |
Unbalanced overexpression of the mutant allele in murine Patched mutants. | ||||||
Authors |
Calzada-Wack J; Kappler R; Schnitzbauer U; Richter T; Nathrath M; Rosemann M; Wagner SN; Hein R; Hahn H | ||||||
Journal |
Carcinogenesis | ||||||
Volume |
23 | ||||||
Issue |
5 | ||||||
Year |
2002 | ||||||
Pages |
727-33 | ||||||
Abstract |
Inherited mutations of Patched (PTCH) in the nevoid basal cell carcinoma syndrome (NBCCS) lead to several developmental defects and contribute to tumor formation in a variety of tissues. PTCH mutations have been also identified in sporadic tumors associated with NBCCS including basal cell carcinoma (BCC) and medulloblastoma. Mice heterozygous for Ptch recapitulate the typical developmental symptoms of NBCCS and develop rhabdomyosarcoma (RMS) and medulloblastoma. PTCH is assumed to act as a tumor suppressor gene although inactivation of both alleles has been demonstrated only in a fraction of tumors. We have investigated the status of Ptch in RMS of heterozygous Ptch neo67/+ mice. Although the wild-type Ptch allele was retained in tumor tissue, the high levels of Ptch mRNA in these tumors result from overexpression of the mutant Ptch transcript. Our results suggest that the wild-type Ptch allele might be selectively silenced in RMS tissue or, alternatively, that haploinsufficiency of Ptch is sufficient to promote RMS formation in mice. | ||||||
Links |
J:77190 – MGI References 12016144 – National Library of Medicine/PubMed |
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Strain Notes
|
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| Strain | Model Name | Treatment Agent(s) | Organ Affected | Frequency | Model Details |
|---|---|---|---|---|---|
| CD-1.129S2-Ptch1tm1Zim/+ | Muscle - Striated - Skeletal rhabdomyosarcoma | Muscle - Striated - Skeletal |
15 |
||
| B6.129S2-Ptch1tm1Zim/+ | Muscle - Striated - Skeletal rhabdomyosarcoma | Muscle - Striated - Skeletal |
0 |