Reference SummaryCarmichael NG, Environ Health Perspect 2000 Jan;108(1):61-5

Title

 The p53 heterozygous knockout mouse as a model for chemical carcinogenesis in vascular tissue

Authors

 Carmichael NG; Debruyne EL; Bigot-Lasserre D

Journal

 Environ Health Perspect

Volume

 108

Issue

 1

Year

 2000

Pages

 61-5

Abstract

 Heterozygous p53 knockout mice were investigated as a potential model for vascular tumor carcinogenesis. Groups of 20 male mice were exposed by gavage for 6 months to the vascular carcinogen urethane at 1, 10, or 100 mg/kg body weight/day. Wild-type and heterozygous p53 knockout control groups were exposed by gavage to the vehicle alone. Another group of 20 male mice received d-limonene by gavage (d-limonene is noncarcinogenic in mice). The high dose of urethane caused early mortality in the majority of mice associated with histopathologic evidence of toxicity and tumors, including a high incidence of benign and malignant vascular tumors, in all animals. At the intermediate dose, toxicity was less marked and 3 of 20 mice had tumors; mice that received the low dose did not have signs of toxicity or neoplasia. The two control groups had no tumors and the d-limonene group had one tumor of the prostate, which was considered spontaneous. We conclude that the p53 knockout mouse is a useful tool for investigating vascular tumorogenesis.

Links

 J:60035 – MGI References
10620525 – National Library of Medicine/PubMed

Strain Notes

Strain Note
B6.129S7 Mice were N5 generation backcross onto C57BL/6 and were purchased from Taconic Farms. Catalog # P53N5-W (wild type).
B6.129S7-Trp53tm1Brd/+ Mice were N5 generation backcross onto C57BL/6 and were purchased from Taconic Farms. Catalog # P53N5-T (heterozygotes).

Models
Strain Model Name Treatment Agent(s) Organ Affected Frequency Model Details
B6.129S7 Blood vessel hemangioma Liver

0
B6.129S7 Blood vessel hemangioma Heart

0
B6.129S7-Trp53tm1Brd/+ Blood vessel hemangioma Liver

0
B6.129S7-Trp53tm1Brd/+ Blood vessel hemangioma Heart

0
B6.129S7-Trp53tm1Brd/+ Blood vessel hemangioma
  • d-limonene
 Liver

0
B6.129S7-Trp53tm1Brd/+ Blood vessel hemangioma
  • d-limonene
 Heart

0
B6.129S7-Trp53tm1Brd/+ Blood vessel hemangioma
  • urethane (ethyl carbamate)
 Liver

0 - 65
B6.129S7-Trp53tm1Brd/+ Blood vessel hemangioma
  • urethane (ethyl carbamate)
 Heart

0 - 5
B6.129S7 Blood vessel hemangiosarcoma Spleen

0
B6.129S7 Blood vessel hemangiosarcoma Liver

0
B6.129S7 Blood vessel hemangiosarcoma Abdominal cavity

0
B6.129S7-Trp53tm1Brd/+ Blood vessel hemangiosarcoma Spleen

0
B6.129S7-Trp53tm1Brd/+ Blood vessel hemangiosarcoma Liver

0
B6.129S7-Trp53tm1Brd/+ Blood vessel hemangiosarcoma Abdominal cavity

0
B6.129S7-Trp53tm1Brd/+ Blood vessel hemangiosarcoma
  • urethane (ethyl carbamate)
 Liver

0 - 40
B6.129S7-Trp53tm1Brd/+ Blood vessel hemangiosarcoma
  • urethane (ethyl carbamate)
 Spleen

0 - 5
B6.129S7-Trp53tm1Brd/+ Blood vessel hemangiosarcoma
  • urethane (ethyl carbamate)
 Abdominal cavity

0 - 5
B6.129S7-Trp53tm1Brd/+ Blood vessel hemangiosarcoma
  • d-limonene
 Abdominal cavity

0
B6.129S7-Trp53tm1Brd/+ Blood vessel hemangiosarcoma
  • d-limonene
 Spleen

0
B6.129S7-Trp53tm1Brd/+ Blood vessel hemangiosarcoma
  • d-limonene
 Liver

0
B6.129S7-Trp53tm1Brd/+ Blood vessel hyperplasia
  • urethane (ethyl carbamate)
 Liver

10
B6.129S7-Trp53tm1Brd/+ Blood vessel hyperplasia
  • urethane (ethyl carbamate)
 Heart

5
B6.129S7-Trp53tm1Brd/+ Forestomach hyperplasia
  • d-limonene
 Forestomach

85
B6.129S7 Leukocyte lymphoma Thymus

0
B6.129S7-Trp53tm1Brd/+ Leukocyte lymphoma Thymus

0
B6.129S7-Trp53tm1Brd/+ Leukocyte lymphoma
  • d-limonene
 Thymus

0
B6.129S7-Trp53tm1Brd/+ Leukocyte lymphoma
  • urethane (ethyl carbamate)
 Thymus

0 - 15
B6.129S7-Trp53tm1Brd/+ Liver hepatocellular carcinoma Liver

0
B6.129S7-Trp53tm1Brd/+ Liver hepatocellular carcinoma
  • d-limonene
 Liver

0
B6.129S7-Trp53tm1Brd/+ Liver hepatocellular carcinoma
  • urethane (ethyl carbamate)
 Liver

0 - 20
B6.129S7 Liver hepatocellular carcinoma Liver

0
B6.129S7-Trp53tm1Brd/+ Liver hyperplasia - oval cell
  • urethane (ethyl carbamate)
 Liver

0 - 10
B6.129S7-Trp53tm1Brd/+ Liver hyperplasia - oval cell
  • d-limonene
 Liver

0
B6.129S7-Trp53tm1Brd/+ Liver hyperplasia - oval cell Liver

0
B6.129S7 Liver hyperplasia - oval cell Liver

0
B6.129S7 Lung adenoma Lung

0
B6.129S7-Trp53tm1Brd/+ Lung adenoma Lung

0
B6.129S7-Trp53tm1Brd/+ Lung adenoma
  • urethane (ethyl carbamate)
 Lung

0 - 25
B6.129S7-Trp53tm1Brd/+ Lung adenoma
  • d-limonene
 Lung

0
B6.129S7-Trp53tm1Brd/+ Mesodermal cell/mesoblast sarcoma Subcutis

0
B6.129S7-Trp53tm1Brd/+ Mesodermal cell/mesoblast sarcoma
  • d-limonene
 Subcutis

0
B6.129S7-Trp53tm1Brd/+ Mesodermal cell/mesoblast sarcoma
  • urethane (ethyl carbamate)
 Subcutis

0 - 5
B6.129S7 Mesodermal cell/mesoblast sarcoma Subcutis

0
B6.129S7-Trp53tm1Brd/+ Prostate gland adenoma Prostate gland

0
B6.129S7 Prostate gland adenoma Prostate gland

0
B6.129S7-Trp53tm1Brd/+ Prostate gland adenoma
  • urethane (ethyl carbamate)
 Prostate gland

0
B6.129S7-Trp53tm1Brd/+ Prostate gland adenoma
  • d-limonene
 Prostate gland

5
B6.129S7-Trp53tm1Brd/+ Prostate gland hyperplasia - focal Prostate gland

20
B6.129S7 Prostate gland hyperplasia - focal Prostate gland

0
B6.129S7-Trp53tm1Brd/+ (Unspecified organ) tumor (Unspecified organ)

0
B6.129S7 (Unspecified organ) tumor (Unspecified organ)

0
B6.129S7-Trp53tm1Brd/+ (Unspecified organ) tumor
  • urethane (ethyl carbamate)
 (Unspecified organ)

0 - 100
B6.129S7-Trp53tm1Brd/+ (Unspecified organ) tumor
  • d-limonene
 (Unspecified organ)

5