Reference SummaryYou S, Cancer Cell 2024 Aug 12;42(8):1415-1433.e12

Title

Lymphatic-localized Treg-mregDC crosstalk limits antigen trafficking and restrains anti-tumor immunity.

Authors

You S; Li S; Zeng L; Song J; Li Z; Li W; Ni H; Xiao X; Deng W; Li H; Lin W; Liang C; Zheng Y; Cheng SC; Xiao N; Tong M; Yu R; Huang J; Huang H; Xu H; Han J; Ren J; Mao K

Journal

Cancer Cell

Volume

42

Issue

8

Year

2024

Pages

1415-1433.e12

Abstract

The tumor microenvironment (TME) has a significant impact on tumor growth and immunotherapy efficacies. However, the precise cellular interactions and spatial organizations within the TME that drive these effects remain elusive. Using advanced multiplex imaging techniques, we have discovered that regulatory T cells (Tregs) accumulate around lymphatic vessels in the peripheral tumor stroma. This localized accumulation is facilitated by mature dendritic cells enriched in immunoregulatory molecules (mregDCs), which promote chemotaxis of Tregs, establishing a peri-lymphatic Treg-mregDC niche. Within this niche, mregDCs facilitate Treg activation, which in turn restrains the trafficking of tumor antigens to the draining mesenteric lymph nodes, thereby impeding the initiation of anti-tumor adaptive immune responses. Disrupting Treg recruitment to mregDCs inhibits tumor progression. Our study provides valuable insights into the organization of TME and how local crosstalk between lymphoid and myeloid cells suppresses anti-tumor immune responses.

Links

J:352967 – MGI References
39029466 – National Library of Medicine/PubMed

Models

Strain Model Name Treatment Agent(s) Organ Affected Frequency Model Details
C57BL/6J-ApcMin/+ Intestine - Large Intestine - Colon tumor Intestine - Large Intestine - Colon

observed - 100

C57BL/6-Apctm1Tyj/+ Tg(Vil1-cre)997Gum Intestine - Large Intestine - Colon tumor Intestine - Large Intestine - Colon

observed

C57BL/6-ApcMin/+ Foxp3tm3(HBEGF/GFP)Ayr/+ Intestine - Large Intestine - Colon tumor Intestine - Large Intestine - Colon

observed

C57BL/6-ApcMin/+ Foxp3tm3(HBEGF/GFP)Ayr/+ Intestine - Large Intestine - Colon tumor
  • Diptheria Toxin (DTX)
Intestine - Large Intestine - Colon

observed

C57BL/6-ApcMin/+ Tcratm1Mom Intestine - Large Intestine - Colon tumor Intestine - Large Intestine - Colon

observed - 100

C57BL/6-ApcMin/+ Tcratm1Mom Intestine - Large Intestine - Colon tumor
  • Diptheria Toxin (DTX)
Intestine - Large Intestine - Colon

18

C57BL/6-ApcMin/+ Ccl22em2Tshu/+ Intestine - Large Intestine - Colon tumor Intestine - Large Intestine - Colon

observed

C57BL/6J-ApcMin/+ Intestine - Large Intestine - Colon tumor
  • anti-mouse MHC Class II (M5/115.15.2)
Intestine - Large Intestine - Colon

observed

C57BL/6-ApcMin/+ Ccr4em2Gpt/+ Intestine - Large Intestine - Colon tumor Intestine - Large Intestine - Colon

observed - 89

C57BL/6-ApcMin Ccr4em2Gpt Intestine - Large Intestine - Colon tumor Intestine - Large Intestine - Colon

observed - 56

C57BL/6-ApcMin/+ Ccr4em1Gpt Intestine - Large Intestine - Colon tumor Intestine - Large Intestine - Colon

100

C57BL/6-ApcMin/+ Ccr4em1Gpt Foxp3tm4(YFP/icre)Ayr/+ Intestine - Large Intestine - Colon tumor Intestine - Large Intestine - Colon

33

C57BL/6J-ApcMin/+ Intestine - Small Intestine tumor Intestine - Small Intestine

observed - 100

C57BL/6-ApcMin/+ Tcratm1Mom Intestine - Small Intestine tumor Intestine - Small Intestine

observed - 100

C57BL/6-ApcMin/+ Tcratm1Mom Intestine - Small Intestine tumor
  • Diptheria Toxin (DTX)
Intestine - Small Intestine

100

C57BL/6-ApcMin/+ Ccr4em2Gpt/+ Intestine - Small Intestine tumor Intestine - Small Intestine

observed - 100

C57BL/6-ApcMin Ccr4em2Gpt Intestine - Small Intestine tumor Intestine - Small Intestine

observed - 100

C57BL/6-ApcMin/+ Ccr4em1Gpt Intestine - Small Intestine tumor Intestine - Small Intestine

100

C57BL/6-ApcMin/+ Ccr4em1Gpt Foxp3tm4(YFP/icre)Ayr/+ Intestine - Small Intestine tumor Intestine - Small Intestine

100

C57BL/6J-ApcMin/+ Intestine tumor Intestine

observed

C57BL/6-ApcMin/+ Ccl22em2Tshu/+ Tg(Nr4a1-EGFP/cre)820Khog Intestine tumor Intestine

observed

C57BL/6J-ApcMin/+ Intestine tumor
  • anti-mouse MHC Class II (M5/115.15.2)
Intestine

observed

C57BL/6-ApcMin/+ Foxp3tm3(HBEGF/GFP)Ayr/+ Intestine tumor Intestine

observed